Microbiome

Dear Friends,

Over the years research has shown a powerful connection between the gut and brain, some call the GI tract the second brain.

Anywhere from 40 to 90 percent of children with autism suffer from gastrointestinal symptoms (Schmidt C. 2015).

An over use of antibiotics can lead to overgrowth of the Clostridial genus, and overgrowth of Clostridia is indicated in autism and even schizophrenia (Shaw, 2010).

A compound identified as 3-(3-hydroxyphenyl)-3-hydroxypropionic acid (HPHPH), which is produced by multiple species of anaerobic bacteria of the Clostridium genus, creates gut inflammation and depletes brain catecholamines.

Add to the above an overuse of other medications such as cortico-steroids, bad dietary patterns and too much stress, and the results you get are a weaken gastrointestinal immune system, and a leaky gut—a viscious cycle of chronic inflammation.

  • Schmidt C. 2015. Mental Health: Thinking from the Gut. Nature; 518:512-515
  • Shaw W. 2010. Increased urinary excretion of a 3-(3-hydroxphyenyl)-3-hydroxypropionic acid (HPHPH), an abnormal phenylalanine metabolite of Clostridia spp. in the gastrointestinal tract, in urine samples from patients with autism and schizophrenia. Nutritional Neuroscience; 12(3):135-143

How can the Supernatant Synbiotic and the LactORN help?

We developed the Supernatant Synbiotic formula to protect against hospital and healthcare generated infections, of which Clostridium difficile is one of the leading culprits.  C. diff. caused almost half a million infections among patients in the United States in a single year, according to a study released by the CDC (CDC Newsroom Feb. 2015).  And, Clostridial overgrowth is commonly associated with Autism Spectrum Disorder (ASD).

Our Bulgarian scientists selected strains of lactic acid bacteria that have the best hit against C. difficile— those that produced the strongest bacterocins to inhibit the overgrowth of Clostridia. We have had wonderful feedback from doctors using the Supernatant Synbiotic with patients suffering from recurrent ulcerative colitis and from those treating autistic patients.

LactORN, our newest synbiotic, highlights the power of supplying optimal amounts of the bacterial microRNA, which stimulates the innate immune system compromised by stress to be more able to defend the gut against infectious bacteria and virus.  It also recenters the adaptive immune system so that it doesn’t overreact to incompletely digested peptides, xenobiotics, and bacterial endotoxins such as LPS.

So there you have it.  See the dosing pattern in the clinical notes.

Sincerely yours,

Seann Bardell
BioImmersion.com

Clinical Note:

The suggested dosing is as follow:

Supernatant Synbiotic one capsule a day or as directed by your doctor.

The LactORN Synbiotic- two level teaspoons a week dissolved in the mouth or as directed by your doctor.

Green Facts

Globe_Home 3

The Marion Institute is very special.  It is a root cause solution based non-profit that acts as an incubator for programs and seridipity projects.

Round the Bend Farm- a Center for Restorative Community is one of their important projects, and Desa Van Laarhoven is its director.  Hear her keynote speach—Desa’s story.

Dear Friends,

Do you  know that blueberries are a rich source of fiber? As a matter of fact, bifidobacterium love blueberries and grow well when they’re fed a mixure of the berries.

A repeated-measure, crossover dietary intervention on human volunteers was designed to study the effect of a six week consumption of a low bush blueberry (Vaccinium angustifolium) drink, versus a placebo drink, in modulating the intestinal microbiota.

The results obtained suggest that regular consumption of blueberries can possibly modulate the composition of the intestinal microbiota. The Bifidobacteria appeared to be selectively favored suggesting an important role for the fiber present in low bush blueberries.

Joseph et al. 2007. Fruit polyphenols and their effects on neuronal signaling and behavior in senescence.  Ann NY Acd Sci; 1100:470-85.

Vendrame et al. 2011. Six-week consumption of a wild blueberry powder drink increases Bifidobacteria in the human gut. J. Agric Food Chem; 59(24):12815-20.

Sincerely yours,

Seann Bardell
BioImmersion.com

Clinical Note:

Our Phyto Power contains the 4 species of blueberry, 3 species of rosehip and 4 species of dandelion all from the wilds of Alaskan.  They are wild-crafted (picked in the wild),  and are exceptionally loaded with polyphenols and fiber.

But the berries do more: As we look for ways to reduce age-related decline in neuronal function, or explore methods to retard or reverse the age-related neuronal deficits, we find that the regular consumption of polyphenolics in high active fruits continues to rise to the top of the list. Blueberries are still thought of as one of the very best. The Blueberry Extract along with the Phyto Power is a winning combination.

Take two capsule daily of the Phyto Power.  This is equivalent of eating six of the rosehip (seeds and all), a small hand full of the blueberries, and a small cup of dandelion salad.

Add one capsule of the Blueberry Extract for a boost. This is equivalent of eating a cup plus of blueberries.

Green Facts

Globe_Home 3
“I would like to enter a dialogue with all people about our common home.”  So begins the encyclical delivered to the world last week from Pope Francis.
It was so beautiful.  He spared no words, and here you have the link to the whole talk in its title:  Revolution needed to combat climate change.

Dear Friends,

Beta Glucan Synbiotic:  perfect post antibiotic therapeutic food.

Oral antibiotics are very hard on our army of friendly lactic acid bacteria in our GI tract microbiome.  These friendly bacteria are killed alongside the pathogenic infection, and they must be replaced. The Beta Glucan High Potency Synbiotic is absolutely the perfect choice for this replenishing purpose. And here is why:

It is well understood that the use of antibiotics can strip the GI tract membranes of its probiotic coating of friendly bacteria, leaving this most important one cell layer thick membrane vulnerable to further assault by pathogens.  There is a small window of time where we must recolonize the membrane.

The newer Beta Glucan has a much higher count – it is indeed a potent probiotic. But more than that, it provides a critical component for the colonization- the best kind of fiber!

The fibers are oat bran fiber with its beta glucan fibers, inulin soluble fibers derived from organic chicory root and red beetroot fiber. The oat fiber has only 0.02% oat gluten, and has proven to be very successful with gluten sensitive people.

The beta glucan soluble fibers provide a fibrous home for the good bacteria within the mucous layer, facilitating their rapid reproduction and colonization of the GI tract membrane.

The small amount of inulin provides a favorite and a more readily available fiber-food for the bacteria and is readily consumed by them resulting in their rapid growth and production of the beneficial SCFA (short chain fatty acids) which help in the healing of the GI tracts tight junctions and much more.

The red beetroot provides both soluble and insoluble fiber and the most important flavonoid betalain which is a very powerful antioxidant, anti-inflammatory. It also supports liver function which is important after antibiotic therapy.

The good bacteria are L. acidophilus, L rhamnosus, L. plantarum, S. thermophilus and B. longum.  All wonderful and powerful pedigreed strains that support a healthy GI tract microbiome—See the Beta Glucan technical brief for a more thorough discussion of this wonderful product.

Sincerely yours,

Seann Bardell
BioImmersion.com

Clinical Note:

After your course of antibiotics take one heaping tablespoon of the Beta Glucan High Potency Synbiotic for two weeks. This provides a probiotic count of around 100 billion CFU plus around 5 grams of fiber. It can be taken with a glass of water, or in a smoothie. Kids love the taste. Continue this level of dosing for two weeks, through the window of recolonization, tapering off to a teaspoon after that time.

As you know, because of the LDL cholesterol lowering effect with a two tablespoon daily dose of the beta Glucan, the American Heart Association has given their Healthy Heart Seal of Approval!  One more thing, if you take a tablespoon twice a day before your two largest meals it will slow down the absorption of carbohydrate into the blood stream.  This is great for glycemically challenged people. This is a multi tasking therapeutic food!

Green Facts

Globe_Home 3
“I would like to enter a dialogue with all people about our common home.”  So begins the encyclical delivered to the world last week from Pope Francis.
It was so beautiful.  He spared no words, and here you have the link to the whole talk in its title:  Revolution needed to combat climate change.

Dear Friends,

The world of the human microbiome is a part of the leading edge in medical research today.  Understanding the synergy between our human cells and our human friendly microbial cells is growing rapidly. The goal is achieving better health.

We have taught the importance of adding good probiotic bacteria and more fiber to our diet for over thirty years, with the aim of bridging the gap between the scientific research community and the practicing physician.

The Bioimmersion Therapeutic Food line is still a new medical framework. We use advanced technology that maintains the wholeness of foods for therapeutic purposes. Research demonstrates that foods behave medicinally in the body—repairing, healing, protecting and preventing. Our purpose therefore is to produce concentrated, powerfully nutritious food supplements.

We are very proud and excited to offer you our newest synbiotic formula—LactORN—and to educate you in how it beautifully fits in with our other very concentrated synbiotics, the Beta Glucan High Potency Synbiotic and the Supernatant Synbiotic.

But first here are some helpful definitions:

  • Synbiotic– refers to any nutritional supplement that provides both probiotics and prebiotics.  All three products are synbiotic formulas.  They provide the synergy of pre and probiotics.
  • Beta Glucan– refers to the beta glucan soluble fiber that we derive from oat bran, that delivers many positive benefits. (see benefits below)
  • High Potency– refers to the high count of good bacteria (CFUs) that the Beta Glucan High Potency Synbiotic provides- approximately 100 billion per heaping tablespoon.
  • Supernatant– refers to inactive probiotic cell populations of L. bulgaricus and S. thermophilus and their metabolites.  (Discussed more fully below)
  • LactORN– refers to Lactobacillus oligoribonucleotides.  This is our newest offering and one we are calling the next generation probiotic.  (See discussion below)

The History of BioImmersion Synbiotics

The development of each BioImmersion product represents our working in collaboration and partnership with many scientists, farmers, manufacturing experts and health practitioners from all over the world. Three of our crown jewels in this exciting birthing process are:
Beta Glucan High Potency Synbiotic, Supernatant Synbiotic and LactORN.

The Big Three 3 2

The Beta Glucan High Potency Synbiotic

The content of this product is prebiotic and probiotic— good fibers and good bacteria.

Its fibers are from three sources: organic chicory root, red beet root, and oat bran.  From the chicory we get the soluble fiber inulin which is very bifidogenic, the Bifidobacterium love it, and so do the Lactobacillus—they grow rapidly on it.  Yeast doesn’t.

10% of the oat bran is composed of the soluble beta glucan fibers.  They are a very viscous fiber and when taken with food will lower the glycemic load of the meal.  An obvious good thing for sugar challenged individuals.  Beta glucan fibers prime the GI tract immune system, they support in the prevention of mutation and cancer (Mantovani 2007), they lower bad cholesterol (LDLs), they create a matrix home for the good Lactic Acid Bacteria.

The organic red beet root fibers are both soluble and insoluble.  The soluble fiber are broken down into short chain fatty acids (SCFAs) and the insoluble fiber stimulate peristalsis.  Plus the red beet root supplies betalain, a very powerful anti-inflammatory and antioxidant.

The fermentation of the soluble fibers by the accompaning good bugs produces SCFAs. Butyric acid is use by the lining cells of the colon for energy and facilitating a healthy GI tract cell turnover which supports the prevention of leaky gut syndrome.  The propionic acid helps to curb the apetite thereby support weight loss.

Now let’s look at its good pedigreed bacteria.  They are Lactobacillus acidophilus, Lacto-bacillus rhamnosus, Lactobacillus plantarum, Strep. thermophilus and Bifido longum.  This powerful collection of good bacteria we call our American Collection of Pedigreed strains. They are ATCC identified strains.  ATCC is the largest repository for good bacteria in the world.  Click here—Beta Glucan High Potency Synbiotic—for more on these good bacteria.

The Supernatant Synbiotic Formula

The content of this product is good bugs and supernatant—with just a little bit of fiber.

The good bacteria are from our Bulgarian Collection of Pedigreed Strains and they are:
Lactobacillus acidophilus (multi-strains), Lactobacillus casei, Lactobacillus bulgaricus, Strep. thermophilus and Bifidobacterium longum (multi-strains).

To design this product, we worked together with our Bulgarian scientists. The research behind this product focused on helping hospital patients protect themselves from hospital generated infection like C. diff. caused colitis.

It took our Bulgarian team 3/4 of a year to trial for the strains with the best hit against Clostrium and other problematic organisms.  This has been a tremendously successful product as it provides a specific protection against C. diff and general protection, as well as providing digestive enzymes, B vitamins, bacteriocins, biosurfactants, and protective colonizing ability.  Inulin is used as a carrier for these good bacteria

What is supernatant?  It is the byproducts of bacterial fermentation that are normally disgarded when growing good bugs.  After growing and freeze drying our good bacteria for this product, we then take fresh broth and put a new culture of L bulgaricus and S. thermophilus in it.  This time we collect their metabolites and freeze dry them.  We then arrest the life of these two producing bacteria and mix the now inactiveated whole cells back in with their metabolite. This whole mixture is what we call supernatant. The whole dead cells stimulate in a good way the awakening of the GI tract immune cells; and, of course, the rest of the metabolites can benefit us right from the get-go of taking this product.

For more on this product in the words of my lead Bulgarian scientist click here.

LactORN: Lactobacillus ORNs

This is the next generation probiotics.  It fits within the probiotic family, yet it provides a very different function and prupose.

The focus of this product is the delivery of the Lactobacillus ORNs into the oral cavity.

LactORN provides Lactobacillus casei plus its oligoribonucleotides (ORNs). We added the prebiotic inulin (derived from organic chicory root).

What are the ORNs and why are they the “the next generation probiotic”?

ORNs are the RNA fragments of varying lengths that naturally resides within the cytoplasm of all bacteria.  When a bacteria encounters a new environment like our mouth it releases its ORNs into the oral cavity.  This sends a message to the releasing bacteria to immediately divide and multiply in this new environment.  The ORNs themselves are stimulatory to our innate immune system in our mouth.

ORNs help our immune system to be more alert to possible invasion by viral or bacterial pathogens, but they also help protect our immune system for overreacting.  They help in the restoration of an immune system compromise by stress.

The optimal amount of ORNs is around 30 AUs (roughly comparible to 900 micrograms).  A cup of raw milk provides around 30 AUs of ORNs from the bacteria that is naturally present.  By the way, this product plus all of our synbiotic products are totally dairy and soy free.

Now, why the next generation?  As I said above when we grow probiotics organisms for supplementation, normally the supernatant is not retained.  In the case of our Supernatant Synbiotic we do put back the supernatant but the ORNs are still damaged in the process so they don’t have the same stimulatory effect as freshly released ORNs which is what the LactORN product provides.

We have stayed true to the pioneering work of Marshall (2007) who researched the beneficial use of Lactobacillus casei oligoribonucleotides for over 20 years in many families of animal groups, from shrimp to fish, mice, pig, cows, and humans.

ORNs are a very important new ingredient to be aware of for its powerful effect on the immune system.

For more on the LactORNs click here.

Between the Supernatant Synbiotic and the Beta Glucan High Potency Synbiotic you are offering to your GI tract microbiome 12 different strains of good bacteria.  By adding the LactORN at a dose of two level teaspoons a week you are supporting the maintenance of a strong immune system.  They all work beautifully together or individually as needed.

  • Mantovani et al. (2007). Beta Glucans in promoting health: PRevention against mutation and cancer. Mutation Research:658(3);154-161.
  • Marshall W. (2007). Oligoribonucleotides alert the immune system of animals to the imminence of microbial infections. US patent 7,189,834 B2

Sincerely yours,

Seann Bardell
BioImmersion.com

Clinical Note:

Diversity of good GI tract flora is important. It helps our microbiome resistant phage attacts and the assaults of other pathogens. Diversity of probiotics offer multi-faceted benefits—helping with our digestion, protecting our GI tract membrane, keeping bad population of possible gut microbe at bay, adding genomic diversity and functionality to our body at large, helping with weight management, and more.

Green Facts

Globe_Home 3New Additions to The Tree of LifeDid you know that the biomass of the microbial world exceeds that of the plant and animal world combined (Hogan CM. 2010).  Click on the title above and see the amazing array of colored photos of newly discovered microbial creatures from the oceans of the world.  They too are effected by warming seas.

Activating Probiotics

May 21, 2015

Dear Friends,

How do ORNs “activate” probiotics and modulate the immune system of animals and humans?

The LactORNs replace a nutritional ingredient lost in the Western diet due to all the processing of foods that we eat. Our bodies have evolved with ORNs from bacteria as an important component of our diet.  ORNs released in our mouth activate probiotics to grow and stimulates and balances our immune systems.  This has been shown in the immune systems of all animals tested—from shrimp to fish, mice, rats, pigs, cows, and humans (Marshall, 2007).

As we have learned last week, our diets lack in sufficient amounts of ORNs because we do not eat fermented foods. Furthermore, most of the yogurts and fermented vegetables available to us lack ORNs. This means that we are seriously deficient in this important nutritional element that helps our bodies fight infection.

All bacteria contain ORNs. Marshall (2007) has discovered that ORNs are small fragments of RNA weighing <10,000 Daltons. They are released immediately when bacteria are transferred from acidic culture media into saliva or pH neutral buffer.

Mice fed free <10kDaONRs released by L. casei, prior to a lethal injection of lipopolysaccharide (LPS) are protected from toxic shock death.

Exposure to <10kDaORNs stimulates innate and adaptive immune responses in hosts as diverse phylogenetically as teleosts and mammals.

In-vivo studies with shrimp and cattle show protection against infections when fed with <10kDaORNs released by Generally Recognized As Safe (GRAS) bacteria and yeasts.

The co-evolutionary release and immune modulation by exRNAs (external RNAs) appears to be well-conserved among the microbial and mammalian kingdoms.

Diets of pasteurized and processed foods do not provide sufficient amounts of GRAS organisms to release the exRNAs that modulate an immune response, nor do typical probiotics or commercial yogurts.

Since the discovery that eukaryotic cells release exRNAs that regulate expression of their genes as well as those of nearby cells, we felt it reasonable that a similar phenomenon might exist in bacteria and that the <10kDaORNs were exRNAs or transcriptional factors that control expression of their growth cycle genes.

Through co-evolution, mammalian immune cells adapted a response to them.  In-vitro studies in humans as well as in-vivo studies in mice, shrimp, fish and cattle fed <10kDaORNs released by GRAS organisms significantly increased their protection against infection and the lethality of septic shock.

The L. casei doesn’t have to be cultureable but remains viable but if it is and enters a human mouth with a neutral pH of 7 then the bacteria accomplishes two things:  it will release its ORNs which stimulates the oral immune system to respond, and two, the release of ORNs causes an immediate cell division by those bacteria for whom the release of ORNs has occurred when they are returned to an environment rich in nutrients.

If all the bacteria have not released their ORNs in the mouth, those that didn’t will release them when they again reach an area with an alkaline pH such as the small intestines.  They start to divide and multiply with no lag time.  If the pH of the mouth is more acidic than 5, only 1/3 of ORNs will be released.  One can add the LactORNs to a baking soda drink (the formula can be found on the back of a box of Arm and Hammer baking soda and gargle to insure that all LactORNs are released.

There is no toxicity to the ORNs.  They restore the immune system without causing chronic inflammation.  The cytokines released are IL-1, IL-6, IL-4 and TNF alpha.  To IL-1 and 6 control inflammation and IL-4 activates B-cells and antibody production.  IL-6 seems to protect the body from too much inflammation.  Overall low level of inflammation are incited to prepare the immune system for a more effective prevention of infection from bacteria and virus.

Dosage:  Individuals taking prescription drugs should inform their doctors before taking LActORNS.  A restored immune systme may be more sensitive  to  the drugs and the prescription might need to be changed.   For example statins may be need to be lowered or eliminated and HBP medication may need to be reduced as well as blood thinners.

Marshall W. 2007. Oligoribonucleotides alert the immune system of animals to the imminence of microbial infections. US patent 7,189,834 B2

LactORNs 3

Sincerely yours,

Seann Bardell
BioImmersion.com

Clinical Note:

Our LactORN is the only probiotic supplement in the market that specifically grows the ORNs. Take one level teaspoon two to five times a week, depending on the weakness of the immune system. Continue to take the one of the probiotics mixes for a diverse microbial support of the GI, such as Original, No 7, or Supernatant. Add fibers such as the Beta Glucan or Energy Sustain for even a greater nutritional support.

LactORN Bottle
ORN Supplemental Facts 2

Green Facts

Here’s more proof as to diet’s influence over our maintaining a healthy microbiome throughout our life. Just look at the elderly.

Claesson et al. (2012) state that, the gut microbiota composition correlates with diet and health in the elderly.  Their microbiota composition significantly correlates with measures of frailty, co-morbidity, nutritional status, markers of inflammation and with metabolites in faecal water.  The individual microbiota of people in long-stay care was significantly less diverse than that of community dwellers.  Collectively, the data support a relationship between diet, microbiota and health status, and indicate a role for diet-driven microbiota alterations in varying rates of health decline upon aging.

Claesson et al. (2012). Gut microbiota composition correlates with diet and health in the elderly. Nature;488:178-184